First Step™ Oral Fluid Drug Screen Device

The Oral Fluid Drug Test is a lateral flow chromatographic immunoassay for the qualitative detection of drugs and drug metabolites in oral fluid. It is based on the principle of competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody. 

During testing through First Step, a portion of the oral fluid specimen migrates along the test strip by capillary action. A drug, if present in the oral fluid specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible coloured line will show up in the test line region of the specific drug strip. 

The presence of drug above the cut-off concentration in the oral fluid specimen will saturate all the binding sites of the antibody. Therefore, the coloured line will not form in the test line region. A drug-positive oral fluid specimen will not generate a coloured line in the specific test line region of the strip because of drug competition, while a drug-negative oral fluid specimen will generate a line in the test line region because of the absence of drug competition. To serve as a procedural control, a coloured line will always appear at the control line region, indicating that a proper volume of specimen has been added and membrane wicking has occurred.

• Easy, fast, and accurate saliva drug test.
• Less invasive than urine.
• Test anywhere – anytime.
• From collection to results in 10 minutes.
• Tests for drugs as per AS/NZS 4760:2019.
• Tests for Amphetamine, Benzodiazepine, Cocaine, Marijuana, Methamphetamine, Opiates, Oxycodone, 
• Donor swabs mouth under supervision until indicator turns red.
• Built-in saturation indicator for assurance enough saliva has been collected for the test to run

First Step Oral Fluid Drug Screen Procedures

1. Bring the pouch to room temperature before opening it. Remove the First Step Product test device from the sealed pouch and use it as soon as possible. Remove the collection swab from packaging. Start Timer. Relax the mouth and insert the collection swab into the mouth. The collection swab must be horizontal throughout the collection process. Using a circular motion, gently swab both cheeks 5-10 times, gums 5-10 times, and surface of tongue 5-10 times, actively swab the inside the mouth, top of tongue, and between cheek and gum until a red colour on the saturation indicator strip appears in the indicator window of collection swab.
2. Remove the test device from the sealed pouch and place upright on a clean, flat surface. Gently and slowly insert the collection swab into the test device, sponge first, until it reaches the bottom of the test device. Push the cap until it locks in place and is secure. (See illustration 2) Important: Keep test device upright while inserting collection swab. Once the collection swab is locked in place, the test device is airtight, tamper evident and ready to be shipped to a lab for confirmation if required. Alternatively, the test device can be disposed of.
3. Keep the test device upright on a flat surface until the test is complete. Start timer. Important: If any test strips do not develop (invalid), peel away the bottom of the device label to inspect specimen volume.
4. Interpret results in 10 minutes.

Test Cut-Off Levels

• Amphetamine AMP: 50ng/mL
• Benzodiazepines BZO: 50ng/mL
• Cocaine COC: 50ng/mL
• Marijuana THC: 15ng/mL
• Methamphetamine METH: 50 ng/mL
• Opiate OPI: 50ng/mL
• Oxycodone OXY: 40ng/mL

Important: Do not bite, suck or chew on the collection swab. It is critical that the collection swab is held horizontally during collection otherwise there will be insufficient saliva collected although the indicator turns red. During collection of the oral fluid, relax the mouth while swabbing the tongue and cheek as this will aid in the collection of the oral fluid.

Verified as Compliant with AS/NZS 4760:2019. Certificate available upon request.

Boxes of 25

Orders made before 12pm will be shipped on the same day. Delivery times will vary. Upon dispatch of your order, we will send you an email containing the tracking number for you to monitor your purchase.

The freight charges are determined by the number of tests ordered and the delivery location. We consistently ship orders at the lowest possible cost using our reliable courier services. Below are the anticipated delivery schedules.

Capital Cities – Next day delivery (up to 5pm next day)

Regional Areas – Two to three days

Remote Areas – Five days

Need your order urgently? Inquire about our expedited courier options. Call 1800 187 238.

When an order is placed prior to 12pm local EST time, below are the expected delivery times. Once an order has been dispatched we will email you the tracking number so you can track your purchase.

Capital Cities – Next day delivery (up to 5pm next day)
Regional Areas – Two to three days
Remote Areas – Five days

Need your order urgently? Ask us about express courier options. 1800 187 238